Quiz: Affinity chromatography

Multiple choice: Check your answers by clicking on them.

1. Affinity chromatography separation is based on…
… the duration of the analyte
… molecular weights
… specific interaction between the analyte and the ligand
… the flow-through time of the analyte
2. Affinity Chromatography can be used for…
… the determination of protein molecular weights
… the separation of proteins according to their isoelectric points
… the concentration of a compound
… the study of enzyme-substrate interactions
… the purification of compounds from a complex matrix
3. Requirements for affinity chromatography…
… ligand affinity to the sample matrix
… highly concentrated solution of the target analyte
… possibility to attach the ligand to the stationary phase
… specific and stable ligand
… reversible bond between ligand and target analyte
… availability of high molecular weight ligand
… degradability of the analyte
4. A pull down affinity chromatography assay is used for the investigation of …
… adjustment of protein charges
… folding of denatured proteins
… protein-protein interactions
… detection of bait proteins

 

Advertisements