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Polarity and Solubility
The content below conveys a basic understanding of (click on the “G” to view the glossary entry): intramolecular forces (G) (electronegativity (G), valence electrons (G), chemical bonds (G)) intermolecular forces (G) (dipole-dipole interaction (G), ion-dipole interaction (G) and dipole-induced dipole interaction (G)) factors influencing a molecules solubility (e.g. pH (G), logD/logP (G)) classification of solvents according … Continue reading Polarity and Solubility
Sampling and Sample Preparation
This lesson points out important things to consider when taking samples. It also conveys a basic understanding of the sample preparation processes (e.g. lyophilization (G), homogenisation (G), solid phase extraction (G), liquid-liquid extraction (G) etc.) and the downstream analytical methods and modes of sample detection (e.g. gas chromatography (G), mass spectrometry (G), liquid chromatography (G), etc.). Analytical errors … Continue reading Sampling and Sample Preparation
Chromatography
Content (click on the “G” to view the glossary entry): Defintions and Separation Mechanisms, Stationary phases, (G), Mobile Phases (G) and Elution (G) Chromatographic parameters and van Deemter Equation (G) Normal Phase Chromatography (G) Reversed Phase Chromatography (G) Size Exclusion Chromatography (G) Ion Chromatography (G) Affinity Chromatography (G) Gas Chromatography (G) Supercritical Fluid Chromatography Hydrophobic Interaction … Continue reading Chromatography
Mass Spectrometry
The following lesson conveys a basic understanding about mass spectrometry (G). It is structured into subdivisions: MS basics including terminology, MS resolution and MS accuracy MS sample inlet and MS ion sources (introduction of different types of ion sources and the modes of ionization) MS analyzers and detectors Basics: Sample inlet and ion source: Mass analyzer … Continue reading Mass Spectrometry
Workflows
This lesson teaches you how to find, indentify and quantify your target. Some basic workflow strategies are being discussed. Basics: Target quantification: Suspected-target Screening: Non-target Screening:
Bioanalytical methods
This chapter includes slides about (click on the “G” to view the glossary entry): Basic knowledge required for the understanding of bioanalytical methods (detergents (G) and the formation of detergent micelles & critical micellar concentration (G) (CMC), isoelectric point) Electrophoresis (Northern Blot (G)/ Southern Blot (G)/ Western Blot (G), SDS Page (G) and Native Page (G), Isoelectric focussing … Continue reading Bioanalytical methods
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