Chromatography

Content (click on the “G” to view the glossary entry):

  • Defintions and Separation Mechanisms,
  • Stationary phases, (G), Mobile Phases (G) and Elution (G)
  • Chromatographic parameters and van Deemter Equation (G)
  • Normal Phase Chromatography (G)
  • Reversed Phase Chromatography (G)
  • Size Exclusion Chromatography (G)
  • Ion Chromatography (G)
  • Affinity Chromatography (G)
  • Gas Chromatography (G)
  • Supercritical Fluid Chromatography
  • Hydrophobic Interaction Chromatography

Chromatographic Basics

Definitions and separation mechanisms:

Elution:

Reversed and Normal Phase Chromatography

Normal phase chromatography:

Reversed phase chromatography:

Normal and reversed stationary phases:

Two-dimensional Chromatography

Some further information about liquid chromatography provided by Agilent Technologies:

© 2015 Agilent Technologies Inc., all rights reserved, for teaching purpose only, used with permission

HPLC Application Notes provided by KNAUER:

© 2011 KNAUER Wissenschaftliche Geräte GmbH, all rights reserved, used with permission

HPLC guide provided by Phenomenex LTD. (german):

© Phenomenex Inc., all rights reserved, used with permission

Affinity Chromatography

Further information provided by GE Healthcare:

Affinity Chromatography – Handbook

© 2007 GE Healthcare Bio-Sciences AB, all rights reserved, used with permission

Ion Chromatography

Further information provided by Metrohm:

© 2018 Deutsche METROHM GmbH & Co. KG, all rights reserved, for teaching purpose only, used with permission

Further information provided by GE Healthcare:

Supercritical Fluid Chromatography

Further information published by Bieber and Letzel, provided by Agilent Technologies:

© 2015 Agilent Technologies Inc., all rights reservied, used with permission

Size Exclusion Chromatography

Further information provided by Agilent Technologies:

Introduction to SEC

© 2014 Agilent Technologies Inc., all rights reserved, used with permission

Hydrophobic Interaction Chromatography

Gas Chromatography

Further information provided by Agilent Technologies:

© 2015 Agilent Technologies, all rights reserved, for teaching purpose only, used with permission

Hydrophilic Interaction Chromatography

Solutions of Greco and Letzel provided by Separation Science:

 

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